Journal: Cell reports

Article Title: Dissecting the Roles of GABA and Neuropeptides from Rat Central Amygdala CRF Neurons in Anxiety and Fear Learning

doi: 10.1016/j.celrep.2019.08.083

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Guinea pig polyclonal anti-Dynorphin , Neuromics , Cat# GP10110; RRID:AB_1621439.

Techniques: Virus, Recombinant, RNAscope, Multiplex Assay, cDNA Synthesis, Gene Expression, Bicinchoninic Acid Protein Assay, Software

Journal: PLoS ONE

Article Title: Adenosine A 2A Receptors in Striatal Glutamatergic Terminals and GABAergic Neurons Oppositely Modulate Psychostimulant Action and DARPP-32 Phosphorylation

doi: 10.1371/journal.pone.0080902

Figure Lengend Snippet: Ambulation was recorded for 180 min after injection of a single dose of cocaine (25 mg/kg, i.p.) or vehicle in fb-A 2A R KO (n = 12, A ) and in st-A 2A R KO (n = 9, B ) mice and their WT littermates (n = 8–12). The arrow indicates the time of injection and the data are mean ± SEM; *p<0.05, comparing fb-A 2A R KO and st-A 2A R KO groups to their corresponding WT group using two-way ANOVA and a post hoc Bonferroni test. ( C ) Cocaine-induced c-Fos expression in the striatum of fb-A 2A R KO (n = 12) and st-A 2A R KO (n = 9) and their corresponding WT littermates (n = 8–12) # p<0.05, comparing to corresponding wild-types with cocaine treatment, two-way ANOVA post hoc Bonferroni test. ( D ) Representative co-immunostaining of c-Fos with dynorphin. Black arrows indicate neurons co-stained with dynorphin and c-Fos; white arrow heads indicate neurons stained with dynorphin only (greyish brown) and black arrow heads indicate neurons stained with c-Fos (reddish brown). Scale bar = 25 µm.

Article Snippet: For this procedure, the first antibody, i.e. , a goat anti-dynorphin polyclonal antibody (1:200, sc-46313, Santa Cruz, CA, USA) was detected first, using immunoperoxidase staining enhanced with 0.08% nickel ammonium sulfate, which yields a dark grayish color.

Techniques: Injection, Expressing, Immunostaining, Staining